6PTF
Crystal Structure of CobT from Methanocaldococcus jannaschii in Apo State
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-15 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.010, 56.010, 243.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.097 - 2.203 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l0z |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.100 | 2.282 |
High resolution limit [Å] | 2.203 | 2.203 |
Rmerge | 0.055 | 0.692 |
Number of reflections | 20650 | 1997 |
<I/σ(I)> | 24.59 | 3.15 |
Completeness [%] | 99.7 | 99.35 |
Redundancy | 12.4 | 12.2 |
CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Crystals formed by mixing 13.3 mg/ml protein solution with well solution containing 8.4% PEG 8K, 100 mM MES/ acetate buffer pH 5.5, 1 mM 5-hydroxybenzimidazole. Crystals were cryoprotected by overnight soak in 20% ethylene glycol, 100 mM MES/acetate buffer pH 5.5, 16% Peg 4K and 5 mM 5,6-dimethylbenzimidazole prior to freezing |