6PTA
Crystal structure of the ARF family small GTPase ARF1 from Candida albicans in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-04-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 86.801, 42.285, 90.343 |
Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
Resolution | 24.500 - 2.500 |
R-factor | 0.2594 |
Rwork | 0.257 |
R-free | 0.29480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r8q |
RMSD bond length | 0.005 |
RMSD bond angle | 0.782 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.118 | 0.834 |
Rpim | 0.076 | 0.533 |
Number of reflections | 22742 | 1081 |
<I/σ(I)> | 8.61 | 1.02 |
Completeness [%] | 98.5 | 97 |
Redundancy | 3.2 | 3.2 |
CC(1/2) | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.2 M ammonium Acetate, 30% PEG 4K, 0.1M sodium acetate pH 4.6 |