6PSH

Crystal structure of periplasmic domain of antiholin RI from T4 phage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-04-05
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9795
Spacegroup name	P 62 2 2
Unit cell lengths	49.975, 49.975, 118.677
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.660 - 2.210
R-factor	0.2885
Rwork	0.284
R-free	0.32290
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.731
Data reduction software	TRUNCATE
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.250
High resolution limit [Å]	2.210	6.000	2.210
Rmerge	0.106	0.084	0.700
Total number of observations	122852
Number of reflections	4888	311	240
<I/σ(I)>	6.3
Completeness [%]	99.9	98.7	100
Redundancy	25.1	20.2	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	289	100 mM Na citrate (pH 4.5), 1M Na acetate, 0.5% Anapoe X114