6PRM
Crystal structure of apo PsS1_19B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-05 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.510, 88.580, 106.600 |
| Unit cell angles | 82.22, 79.85, 84.20 |
Refinement procedure
| Resolution | 47.600 - 2.500 |
| R-factor | 0.2393 |
| Rwork | 0.238 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bia |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.426 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.200 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | 0.334 |
| Number of reflections | 58432 | 4508 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 3350, sodium acetate trihydrate |
