6PRL
Assembly of VIQKI P5(beta-L-homoproline) with human parainfluenza virus type 3 (HPIV3) fusion glycoprotein N-terminal heptad repeat domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.521, 53.160, 58.231 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 39.260 - 1.870 |
R-factor | 0.2074 |
Rwork | 0.203 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nro |
RMSD bond length | 0.002 |
RMSD bond angle | 0.437 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.260 | 1.937 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmeas | 0.298 | 3.064 |
Rpim | 0.113 | 1.281 |
Number of reflections | 17950 | 1687 |
<I/σ(I)> | 8.57 | 1.99 |
Completeness [%] | 98.8 | 93.35 |
Redundancy | 6.9 | 4.9 |
CC(1/2) | 0.971 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30 mM NaF; 30 mM NaBr; 30 mM NaI; 20% v/v PEG 500 MME; 10 % w/v PEG 20000; 100 mM imidazole/MES monohydrate (pH 6.5) |