6PRK

X-ray Crystal Structure of Bacillus subtilis RicA in complex with RicF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88557
Spacegroup name	P 62
Unit cell lengths	86.090, 86.090, 87.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.040 - 3.200
R-factor	0.209
Rwork	0.205
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6prh
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmeas	0.085
Number of reflections	6195	312
<I/σ(I)>	17.24
Completeness [%]	99.9
Redundancy	9.95
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% (vol/vol) PEG500 MME, 8% (vol/vol) MPD, 100 mM sodium nitrate, 100 mM MOPS, Sodium HEPES pH 7.3