6PRH

X-ray Crystal Structure of Bacillus subtilis RicA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	93
Detector technology	CCD
Collection date	2016-06-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.18076
Spacegroup name	C 1 2 1
Unit cell lengths	109.491, 52.212, 27.109
Unit cell angles	90.00, 95.83, 90.00

Refinement procedure

Resolution	29.830 - 2.080
R-factor	0.1849
Rwork	0.183
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pih
RMSD bond length	0.010
RMSD bond angle	1.588
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.080	5.640	2.080
Rmerge	0.141	0.107	0.396
Rmeas	0.151	0.115	0.428
Rpim	0.055	0.042	0.159
Number of reflections	9208	484	437
<I/σ(I)>	10.1
Completeness [%]	99.8	99.6	99.1
Redundancy	7.5	7.3	6.9
CC(1/2)	0.990	0.992	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES pH 6.0, 150 mM NaCl, 65% (vol/vol) MPD