6PQB

Crystal structure of aminoglycoside-resistance methyltransferase RmtC bound to S-adenosylhomocysteine (SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 61
Unit cell lengths	163.540, 163.540, 122.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.885 - 3.140
R-factor	0.2018
Rwork	0.200
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cn0
RMSD bond length	0.002
RMSD bond angle	0.474
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	3.250
High resolution limit [Å]	3.140	3.140
Rpim	0.047	0.870
Number of reflections	32552	3224
<I/σ(I)>	10.82	1.15
Completeness [%]	100.0	100
Redundancy	8.7	8.2
CC(1/2)	0.990	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2M ammonium sulfate, 0.1 M Na HEPES pH 7.0, 3 mM mellitic acid