6PPP

Structure of S. pombe Lsm2-8 with processed U6 snRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.078123
Spacegroup name	P 2 21 21
Unit cell lengths	70.260, 139.690, 153.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.700 - 2.330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4emg 4emh 4emk 4c92 4m7d
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.000	2.730
High resolution limit [Å]	2.330	2.330
Number of reflections	28712	1433
<I/σ(I)>	16
Completeness [%]	43.7
Redundancy	86.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	50 mM KCl 50 mM HEPES pH 7 Pentaerythritol propoxylate (5/4 PO/OH)