6PON
CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF FIBRONECTIN- BINDING PROTEIN PAVA FROM STREPTOCOCCUS PNEUMONIAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-04 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.873, 46.814, 83.678 |
Unit cell angles | 90.00, 90.93, 90.00 |
Refinement procedure
Resolution | 41.830 - 2.395 |
R-factor | 0.17 |
Rwork | 0.167 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h3x |
RMSD bond length | 0.008 |
RMSD bond angle | 0.905 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.667 | 2.440 |
High resolution limit [Å] | 2.395 | 2.395 |
Rmeas | 0.110 | |
Rpim | 0.060 | 0.272 |
Number of reflections | 23361 | 2228 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.0 | |
Redundancy | 3.26 | |
CC(1/2) | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 27% (w/v) PEG 4000, 0.2 M Ammonium Acetate, 0.1 M Sodium Citrate pH 5.6 |