6PNW

X-RAY STRUCTURE OF ERABUTOXIN C, A DIMERIC NEUROTOXIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	290
Detector technology	DIFFRACTOMETER
Collection date	1983-02-01
Detector	SYNTEX
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	55.600, 53.750, 40.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
Rwork	0.161
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ebx ERABUTOXIN B
Data reduction software	SORTVA
Data scaling software	SORTAV
Phasing software	TFORM
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.200
High resolution limit [Å]	2.000	2.000
Rmerge	0.080
Number of reflections	7681	986
<I/σ(I)>	7.3
Completeness [%]	85.6	70
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	PHOSPHATE BUFFER, AMMONIUM SULFATE PH 6