6PMC
TRK-A IN COMPLEX WITH LIGAND 1a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9282 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 52.657, 52.657, 233.544 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.640 - 2.190 |
R-factor | 0.25 |
Rwork | 0.246 |
R-free | 0.29840 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.901 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.850 | 2.440 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 18645 | 5039 |
<I/σ(I)> | 15.19 | |
Completeness [%] | 95.1 | 94.4 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.10M KH2PO4/ 0.10M NaH2PO4/ 0.1M MES/NaOH pH = 6.00 and 1.9M NaCl (cryo: 25% glycerol in reservoir), or 18% (w/v) PEG 3350, 0.2M CaCl2, 0.10M, MES pH = 6.50 (cryo: direct) |