6PM8
Protein Tyrosine Phosphatase 1B (1-301), P180A mutant, vanadate bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.298, 88.298, 104.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.660 - 2.060 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qkq |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.660 | 2.134 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.094 | 0.310 |
| Rmeas | 0.099 | |
| Rpim | 0.030 | |
| Number of reflections | 29002 | 2871 |
| <I/σ(I)> | 16.36 | |
| Completeness [%] | 98.3 | 98.22 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Hepes, 0.2 M magnesium acetate and 15-20% polyethylene glycol 8000 |
