6PLN
X-ray crystal structure of Pyrococcus furiosus general transcription factor TFE-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.689, 55.689, 248.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.270 - 2.600 |
R-factor | 0.2444 |
Rwork | 0.239 |
R-free | 0.28650 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.125 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.064 | 0.991 |
Number of reflections | 13000 | 645 |
<I/σ(I)> | 59 | 3.55 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.1 | 12.8 |
CC(1/2) | 0.842 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 10 % PEG 8000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 7) |