6PLL
Crystal structure of the ZIG-8 IG1 homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2019-04-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9791928 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.560, 73.560, 270.631 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.381 - 2.693 |
R-factor | 0.2385 |
Rwork | 0.237 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6on9 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.753 |
Data reduction software | XDS (20190315) |
Data scaling software | XDS (20190315) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.225 | 2.597 |
Rmeas | 0.230 | 2.673 |
Number of reflections | 12889 | 1967 |
<I/σ(I)> | 15.33 | 1.05 |
Completeness [%] | 99.7 | 98.3 |
Redundancy | 21.4 | 17.7 |
CC(1/2) | 0.998 | 0.529 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Ammonium dihydrogen phosphate, 20% (w/v) PEG 3350 |