6PKF

Myocilin OLF mutant N428E/D478K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	45.506, 58.688, 89.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.369 - 1.484
R-factor	0.152
Rwork	0.150
R-free	0.18090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ou1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.369	1.540
High resolution limit [Å]	1.480	1.480
Rmeas	0.129
Rpim	0.039
Number of reflections	40126	41877
<I/σ(I)>	31.73
Completeness [%]	97.6	95.49
Redundancy	11.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	PEG 3350, M Bis Tris pH 6, magnesium formate