6PKE
Myocilin OLF mutant N428E/D478S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.222, 47.294, 97.020 |
| Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.880 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6ou2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.774 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 5.100 | 1.880 |
| Rmerge | 0.117 | 0.055 | 0.459 |
| Rmeas | 0.139 | 0.065 | 0.558 |
| Rpim | 0.073 | 0.033 | 0.312 |
| Total number of observations | 108492 | ||
| Number of reflections | 34580 | 1784 | 1641 |
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 97.8 | 95.9 | 95 |
| Redundancy | 3.1 | 3.2 | 2.8 |
| CC(1/2) | 0.997 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG 8000, MgCl2 |
