6PK5

Adenylate kinase from Methanococcus igneus - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 31
Unit cell lengths	68.710, 68.710, 257.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.332 - 2.300
R-factor	0.2306
Rwork	0.229
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	60464	9674
<I/σ(I)>	9.08	1.04
Completeness [%]	99.8	98.9
Redundancy	5.89
CC(1/2)	0.997	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	30%