6PK1

Alanine-glyoxylate aminotransferase 1 (AGT1) from Arabidopsis thaliana in presence of serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2001-02-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	139.179, 62.376, 96.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.160 - 2.100
R-factor	0.1187
Rwork	0.115
R-free	0.16610
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6pk3
RMSD bond length	0.015
RMSD bond angle	1.132
Data reduction software	DENZO (0.95)
Data scaling software	SCALEPACK (1.6.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.160	22.160	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.100	0.057	0.263
Rmeas	0.110	0.064	0.320
Rpim	0.040	0.029	0.178
Number of reflections	45371	2622	1230
<I/σ(I)>	16
Completeness [%]	90.5	94.4	49.6
Redundancy	5	5.1	2.3
CC(1/2)	0.990	0.989	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	Protein: 11 mg/ml in 20 mM serine, 0.2 mM pyridoxal-phosphate, 10% glycerol, 100 mM Tris-HCl, pH 8.5. Precipitant: 4.1-4.2 M sodium formate, 20 mM serine. One microliter protein + 1 microliter precipitant equilibrated against precipitant.