6PJ9
Time-resolved structural snapshot of proteolysis by GlpG inside the membrane
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.260, 99.000, 63.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.840 - 2.500 |
R-factor | 0.2587 |
Rwork | 0.258 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f5d |
RMSD bond length | 0.006 |
RMSD bond angle | 1.473 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.840 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 7975 | 871 |
<I/σ(I)> | 4.5 | |
Completeness [%] | 99.2 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Na-acetate pH 5.5, 3 M NaCl, and 5 % ethylene glycol |