6PIL
Antibody scFv-M204 monomeric state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.7749 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 60.420, 60.420, 159.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.500 - 2.200 |
R-factor | 0.198 |
Rwork | 0.194 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE (20180126) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.500 | 56.500 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.072 | 0.022 | 0.644 |
Rmeas | 0.084 | 0.027 | 0.752 |
Total number of observations | 55416 | ||
Number of reflections | 15422 | 220 | 1120 |
<I/σ(I)> | 11.14 | 32.34 | 1.91 |
Completeness [%] | 97.9 | 94.4 | 99 |
Redundancy | 3.593 | 3.109 | 3.653 |
CC(1/2) | 0.999 | 1.000 | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.2 M Lithium sulfate, 0.1 M Phosphate/Citrate pH 4.2, 20% PEG 1000 |