6PII
The evolving story of AtzT, a periplasmic binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.008020 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.920, 63.050, 120.436 |
Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
Resolution | 49.400 - 1.870 |
R-factor | 0.15624 |
Rwork | 0.155 |
R-free | 0.18303 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.548 |
Data reduction software | ADDREF |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 1.900 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.140 | 0.669 |
Rpim | 0.032 | 0.246 |
Number of reflections | 119947 | 4833 |
<I/σ(I)> | 13.5 | 3.1 |
Completeness [%] | 97.8 | 80.1 |
Redundancy | 19.6 | 7.4 |
CC(1/2) | 0.998 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein at 20 mg/mL in SD2 plates incubated at 20 C. Conditions contained 6-8% Jeffamine M600 with 2-7% MPD and trisodium citrate at 1.1-1.2 M |