6PHV
SpAga galactose product complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-26 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5419 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 90.634, 127.283, 151.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.770 - 2.100 |
| R-factor | 0.1784 |
| Rwork | 0.176 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6phu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.092 | 0.316 |
| Rpim | 0.042 | 0.189 |
| Number of reflections | 50574 | 3789 |
| <I/σ(I)> | 14.8 | 3.1 |
| Completeness [%] | 97.9 | 91.1 |
| Redundancy | 3.5 | 2.6 |
| CC(1/2) | 0.989 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 1.0 M sodium phosphate monobasic/potassium phosphate dibasic |
