6PHR
Crystal structure of Marinobacter subterrani acetylpolyamine amidohydrolase (msAPAH) complexed with 5-[(3-aminopropyl)amino]pentane-1-thiol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.874, 120.680, 65.360 |
| Unit cell angles | 90.00, 109.09, 90.00 |
Refinement procedure
| Resolution | 61.765 - 1.650 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4ZUM |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.946 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.770 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.106 | 0.675 |
| Number of reflections | 79658 | 7965 |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 10 mg/ml protein, 20% v/v-propanol, 0.1 MES monohydrate (pH 6.0), 20% w/v polyethylene glycol monomethyl ether 2000, 1:1 ratio protein to precipitant |
