6PHP
Crystal structure of glucagon analog with 4-bromo-phenylalanine substitutions at position 6 and 22 in space group I41 at 1.65 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-05-17 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 41 |
Unit cell lengths | 41.712, 41.712, 30.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.490 - 1.650 |
R-factor | 0.2266 |
Rwork | 0.225 |
R-free | 0.24580 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.556 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.490 | 2.010 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmeas | 0.110 | 1.320 |
Rpim | 0.042 | 0.510 |
Number of reflections | 3194 | 1267 |
<I/σ(I)> | 10.7 | 1.1 |
Completeness [%] | 99.7 | |
Redundancy | 6.6 | |
CC(1/2) | 0.996 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tacsimate, pH 4, 16% PEG3350, 0.1 M sodium acetate, pH 4.6 |