6PHM
Crystal structure of glucagon analog fully composed of D-amino acids in space group I41 at 1.1 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-09-20 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | I 41 |
Unit cell lengths | 42.370, 42.370, 28.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.960 - 1.100 |
R-factor | 0.1519 |
Rwork | 0.151 |
R-free | 0.16690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pho |
RMSD bond length | 0.017 |
RMSD bond angle | 1.484 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.960 | 1.290 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmeas | 0.036 | 0.240 |
Rpim | 0.011 | 0.134 |
Number of reflections | 10382 | 3234 |
<I/σ(I)> | 39.3 | 4.1 |
Completeness [%] | 99.3 | 90.2 |
Redundancy | 7.9 | 2.8 |
CC(1/2) | 0.999 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulfate, 20% PEG3350 |