6PHJ

Crystal structure of native glucagon in space group P213 at 1.99 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-05-17
Detector	RDI CMOS_8M
Wavelength(s)	0.97625
Spacegroup name	P 21 3
Unit cell lengths	44.724, 44.724, 44.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.625 - 1.990
R-factor	0.2812
Rwork	0.280
R-free	0.30750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pho
RMSD bond length	0.010
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.630	2.150
High resolution limit [Å]	1.990	1.990
Rmeas	0.056	1.236
Rpim	0.009	0.266
Number of reflections	2188	419
<I/σ(I)>	43.8	2.3
Completeness [%]	100.0	100
Redundancy	32.5
CC(1/2)	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1 M Tris, pH 7