6PHH
Unliganded human transmission blocking antibody 2544
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.530, 43.650, 138.570 |
| Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
| Resolution | 38.976 - 2.400 |
| R-factor | 0.2192 |
| Rwork | 0.218 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house model |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.551 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.131 | 0.724 |
| Rpim | 0.055 | 0.298 |
| Number of reflections | 38316 | 4305 |
| <I/σ(I)> | 11.2 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.996 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M sodium cacodylate HCl, pH 6.5, 1.0 M sodium citrate tribasic |
