6PHG

Predicted germline variant of human transmission blocking antibody 2544

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	130.843, 60.557, 61.079
Unit cell angles	90.00, 113.62, 90.00

Refinement procedure

Resolution	35.782 - 2.000
R-factor	0.1848
Rwork	0.182
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house model
RMSD bond length	0.004
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.599
Rpim	0.034	0.247
Number of reflections	29727	4002
<I/σ(I)>	13.6	1.9
Completeness [%]	99.9	100
Redundancy	6.7	6.7
CC(1/2)	0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium acetate pH 4.5, 25 % (w/v) PEG 3350