6PHD

Pfs25 in complex with the human transmission blocking antibody 2586

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 41 21 2
Unit cell lengths	156.545, 156.545, 85.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.947 - 3.100
R-factor	0.214
Rwork	0.213
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house model
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.293	0.792
Rpim	0.084	0.230
Number of reflections	19905	1770
<I/σ(I)>	8.3	1.7
Completeness [%]	99.9	100
Redundancy	13.1	12.7
CC(1/2)	0.986	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M lithium sulfate, 0.1 M Tris-HCl, pH 8.5, 30 % (w/v) PEG 4000