6PH1

T4 lysozyme pseudo-wild type soaked in TEMPOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.165, 60.165, 95.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.882 - 1.632
R-factor	0.1689
Rwork	0.167
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vnr
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.660
High resolution limit [Å]	1.630	4.420	1.630
Rmerge	0.143	0.090	0.275
Total number of observations	204293
Number of reflections	25545	1398	1213
<I/σ(I)>	33.2		14
Completeness [%]	99.7	99.1	96.9
Redundancy	8	7.7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	298	2.0M Na/K Phosphate pH 6.7, 250mM NaCl