6PH0

T4 lysozyme pseudo-wild type soaked in TEMPO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.656, 59.656, 95.004
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.527 - 1.947
R-factor	0.1636
Rwork	0.162
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vnr
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.010
High resolution limit [Å]	1.947	4.450	1.947
Rmerge	0.151	0.067	0.633
Number of reflections	14827	1355	1216
<I/σ(I)>	8.2		3.4
Completeness [%]	99.7	100	99.3
Redundancy	6.7	6.4	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	2.0M Na/K Phosphate, 250mM NaCl