6PGY
MTSL labelled T4 lysozyme pseudo-wild type K65C mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.020, 61.020, 96.481 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.628 - 2.000 |
R-factor | 0.1594 |
Rwork | 0.158 |
R-free | 0.18170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vnr |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.111 | 0.059 | 0.555 |
Number of reflections | 14595 | 1578 | 1433 |
<I/σ(I)> | 10.3 | 3.5 | |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 6.9 | 6.7 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 2.0M Na/K phosphate |