6PGF
WDR5delta32 bound to N-(4-(4-(hydroxymethyl)-1H-imidazol-2-yl)butyl)acrylamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.819, 65.127, 97.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.710 - 1.540 |
| R-factor | 0.14917 |
| Rwork | 0.148 |
| R-free | 0.16952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pg4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.710 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 43339 | 1913 |
| <I/σ(I)> | 16.2 | 3.2 |
| Completeness [%] | 97.1 | 88 |
| Redundancy | 13.3 | 12 |
| CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.16 M ammonium sulfate, 0.1 M Bis-Tris chloride, pH 6.1, 30.1% w/v PEG8000 |
