6PGE

WDR5delta32 bound to ethyl 3-(4-(hydroxymethyl)-1H-imidazol-2-yl)propanoate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	46.722, 65.180, 97.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.390 - 1.760
R-factor	0.18379
Rwork	0.182
R-free	0.21263
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pg4
RMSD bond length	0.008
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.720	1.790
High resolution limit [Å]	1.760	1.760
Number of reflections	30209	1647
<I/σ(I)>	13.4	2.9
Completeness [%]	99.7	97.5
Redundancy	7.1	7
CC(1/2)	0.997	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.024 M ammonium sulfate, 0.1 M Bis-Tris chloride, pH 5.64, 26.3% w/v PEG4000