6PGD
WDR5delta32 bound to peptidomimetic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.698, 65.240, 97.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.390 - 1.500 |
| R-factor | 0.15153 |
| Rwork | 0.151 |
| R-free | 0.16718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pg4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.408 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 47878 | 1846 |
| <I/σ(I)> | 11 | 2.4 |
| Completeness [%] | 99.0 | 80.5 |
| Redundancy | 7.2 | 6.5 |
| CC(1/2) | 0.997 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.233 M ammonium sulfate, 0.1 M Bis-Tris chloride, pH 6.02, 26% w/v PEG8000 |
