6PG7
WDR5delta32 bound to (2-(3-methoxy-3-phenylpropyl)-1H-imidazol-4-yl)methanol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.963, 65.303, 97.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.650 - 2.450 |
R-factor | 0.19791 |
Rwork | 0.196 |
R-free | 0.24305 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pg4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.403 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.650 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 11523 | 1239 |
<I/σ(I)> | 13.1 | 2.4 |
Completeness [%] | 99.7 | 97.6 |
Redundancy | 6.9 | 6.8 |
CC(1/2) | 0.997 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.17 M ammonium sulfate, 0.1 bis-tris chloride (pH 6.3), 30.5 %w/v PEG4000 |