6PG3

WDR5delta23 bound to (2-butyl-1H-imidazol-4-yl)methanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	I 1 2 1
Unit cell lengths	112.825, 46.850, 129.154
Unit cell angles	90.00, 112.81, 90.00

Refinement procedure

Resolution	43.630 - 2.040
R-factor	0.17459
Rwork	0.173
R-free	0.20617
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h9l
RMSD bond length	0.009
RMSD bond angle	1.392
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.630	2.100
High resolution limit [Å]	2.040	2.040
Number of reflections	39721	2912
<I/σ(I)>	10.3	2.7
Completeness [%]	99.6	94.8
Redundancy	7.4	7.2
CC(1/2)	0.994	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.182 M ammonium fluoride, 0.1 M sodium cacodylate (pH 5.79), 22.1 %w/v PEG8000