6PFY
Membrane Protein Megahertz Crystallography at the European XFEL, Photosystem I at synchrotron to 2.9 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 278.496, 165.126, 283.427 |
Unit cell angles | 90.00, 119.42, 90.00 |
Refinement procedure
Resolution | 48.870 - 2.900 |
R-factor | 0.30537 |
Rwork | 0.303 |
R-free | 0.34306 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jb0 |
RMSD bond length | 0.007 |
RMSD bond angle | 2.260 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.136 | 0.481 |
Number of reflections | 492002 | 24360 |
<I/σ(I)> | 7.1 | 2.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.6 | 3.5 |
CC(1/2) | 0.966 | 0.684 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.4 | 283 | MES 5 mM 5- 30 mM MgSO4 0.02 % beta-DDM |