6PFT

rsEGFP2 with a chlorinated chromophore in the non-fluorescent off-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.305053
Spacegroup name	P 21 21 21
Unit cell lengths	51.014, 62.716, 68.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.309 - 1.450
R-factor	0.1513
Rwork	0.150
R-free	0.17580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dty
RMSD bond length	0.020
RMSD bond angle	1.649
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.309	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.008	0.089
Rmeas	0.012	0.127
Number of reflections	38992	3674
<I/σ(I)>	21.46	5.52
Completeness [%]	98.2	93.61
Redundancy	2	2
CC(1/2)	1.000	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	298	1.76 M ammonium sulfate, 0.1 M HEPES; Cryoprotectant 1 M sucrose, 1.7 M ammonium sulfate, 0.1 M HEPES.