6PFR

rsEGFP2 with a chlorinated chromophore in the fluorescent on-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	50.699, 62.489, 67.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.371 - 1.510
R-factor	0.1889
Rwork	0.188
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtx
RMSD bond length	0.007
RMSD bond angle	0.781
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.371	1.564
High resolution limit [Å]	1.510	1.510
Rmerge	0.012	0.354
Rmeas	0.016	0.500
Number of reflections	32194	3364
<I/σ(I)>	19.5	1.86
Completeness [%]	93.5	99.61
Redundancy	2	2
CC(1/2)	1.000	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	298	1.76 M ammonium sulfate, 0.1 M HEPES; Cryoprotectant 1 M sucrose, 1.7 M ammonium sulfate, 0.1 M HEPES.