6PFQ

Structure of Kluyveromyces marxianus Usb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	69.927, 107.364, 113.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.593 - 1.800
R-factor	0.1936
Rwork	0.192
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.177
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.360	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	80145	4528
<I/σ(I)>	16.3
Completeness [%]	100.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.2 M potassium sodium tartrate, 100 mM HEPES, 30% glycerol