6PEH

Crystal structure of rabbit monoclonal anti-HIV antibody 1C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	71.799, 98.610, 162.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.323 - 2.296
R-factor	0.221
Rwork	0.219
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.717
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.296	2.296
Number of reflections	46339	2060
<I/σ(I)>	12.4
Completeness [%]	88.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Imidazole pH 6.5, 16% PEG 3350, 10% MPD, 0.2M LiSO4