6PDX
Crystal structure of C585 Fab in complex with influenza virus hemagglutinin from A/Switzerland/9715293/2013 (H3N2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-21 |
Detector | RAYONIX MX425-HS |
Wavelength(s) | 1.068 |
Spacegroup name | P 63 |
Unit cell lengths | 287.265, 287.265, 182.933 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.877 - 3.993 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.24150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.830 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.700 | 4.090 |
High resolution limit [Å] | 3.993 | 3.993 |
Number of reflections | 71845 | 4312 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 99.7 | 97.5 |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M sodium cacodylate, 1M sodium citrate |