6PDU
Vaccine-elicited NHP FP-targeting antibody 13N024-a.01 in complex with HIV fusion peptide (residue 512-519)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.032, 74.203, 93.914 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.679 - 1.953 |
R-factor | 0.1744 |
Rwork | 0.173 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
Rmerge | 0.098 | 0.050 | 0.534 |
Rmeas | 0.106 | 0.054 | 0.594 |
Rpim | 0.040 | 0.021 | 0.256 |
Total number of observations | 240628 | ||
Number of reflections | 35653 | 1940 | 1747 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 99.8 | 99.7 | 98.5 |
Redundancy | 6.7 | 6.6 | 5.1 |
CC(1/2) | 0.998 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5M NaCl, 10% PEG 400, 20% PEG 8000, 0.1M Na acetate pH 5.5 |