6PDK
The Structure of Smlt3025, an immunity protein of the Stenotrophomonas maltophilia type IV secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.5419 |
| Spacegroup name | H 3 |
| Unit cell lengths | 67.532, 67.532, 149.386 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.870 - 1.900 |
| R-factor | 0.2003 |
| Rwork | 0.195 |
| R-free | 0.24860 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.050 | 1.910 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19897 | 3140 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M Imidazole/MES Monohydrate pH 6.5 0.03M Sodium Fluoride 0.03M Sodium Bromide 0.03M Sodium Iodide 12.5% MPD 12.5% PEG10000 12.5% PEG3350 |
