6PD0

Crystal structure of the bacterial cellulose synthase subunit G (BcsG) from Escherichia coli, catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	52.510, 76.920, 95.920
Unit cell angles	90.00, 90.58, 90.00

Refinement procedure

Resolution	47.958 - 1.750
R-factor	0.1627
Rwork	0.161
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pcz
RMSD bond length	0.007
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.043	0.266
Rmeas	0.047	0.289
Rpim	0.018	0.113
Number of reflections	74391	6595
<I/σ(I)>	28.56	7.42
Completeness [%]	96.6	85.96
Redundancy	7	6.6
CC(1/2)	0.999	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M HEPES:NaOH, 20% (w/v) PEG 4000, 10% (v/v) 2-propanol