6PCF
Human Coa6: W59C mutant protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.386, 47.823, 48.114 |
| Unit cell angles | 116.86, 98.68, 104.26 |
Refinement procedure
| Resolution | 38.250 - 2.200 |
| R-factor | 0.20273 |
| Rwork | 0.201 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pce |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 15400 | 1521 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH 5.7, 0.23 M MgCl2.6H2O, 29% (w/v) PEG 3350 |
