6PC2
Crystal structure of Helicobacter pylori PPX/GppA in complex with GNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-10 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.016, 94.475, 107.197 |
| Unit cell angles | 90.00, 108.53, 90.00 |
Refinement procedure
| Resolution | 35.884 - 2.900 |
| R-factor | 0.2582 |
| Rwork | 0.254 |
| R-free | 0.30990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hi0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.495 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3352: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.055 | |
| Number of reflections | 23947 | 2375 |
| <I/σ(I)> | 13.8 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2 M Potassium chloride |
