6PBZ
Crystal structure of Escherichia coli GppA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-05 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.829, 162.510, 165.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.461 - 2.475 |
R-factor | 0.2253 |
Rwork | 0.225 |
R-free | 0.27400 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.216 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_3352: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.040 | |
Number of reflections | 78268 | 7470 |
<I/σ(I)> | 13.5 | 0.7 |
Completeness [%] | 97.9 | 94.6 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% (v/v) PEG 3350, 0.1 M HEPES pH 7.5, 0.2 M Magnesium chloride |